ABSTRACT
The present study was carried out on mongrel dogs. Haemorrhagic shock of different severities and duration was produced by exsanguination from an artery. After the required duration of shock, two third of the volume of blood withdrawn was transfused back into the animal. Effect of haemorrhage and reperfusion of blood after haemorrhagic shock on lipid peroxidation was assessed by measuring plasma malondialdehyde (MDA). Severity of shock was assessed from the haematocrit values. There was a significant increase (P < 0.05) in plasma MDA level after blood transfusion in a group having 40 mm Hg blood pressure as magnitude of shock and one hour as duration of shock (Group II) only. Haemotocrit value was also significantly low (P < 0.05) in this group after haemorrhagic shock. Results are suggestive of lipid peroxidation with ischaemic reperfusion in severe and long duration of shock.
Subject(s)
Animals , Blood Transfusion , Dogs , Female , Hematocrit , Lipid Peroxidation/physiology , Male , Malondialdehyde/blood , Shock, Hemorrhagic/metabolism , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
Influence of stress (cold) on visual reaction time (VRT) for red, green and yellow colours and auditory reaction time (ART) for high and low pitched sounds was studied in 60 healthy subjects (17-19 years) by digital display response time apparatus.Pain threshold and pain tolerance time were 23.02 +/- 8.11 and 249.35 +/- 64.88 sec. respectively. During the standard cold pressor test (CPT), there was significant increase (P < 0.01) in VRT for all the three colours, the increase being the least for the red colour. There was significant increase (P < 0.01) in ART for both high and low pitched sounds.There was increase in heart rate and respiratory rate as compared to controls. This increase in VRT and ART with CPT was probably due to decreased conduction velocity of nerves, which could be due to vasoconstriction attributed to increased sympathetic activity caused by cold and pain.
Subject(s)
Adolescent , Adult , Cold Temperature , Hearing/physiology , Heart Rate , Humans , Pain Threshold , Reaction Time , Respiration , Vision, Ocular/physiologyABSTRACT
The study was undertaken to investigate the effect of infusion of isotonic saline of different pH (2, 3, 9 and 10) and different temperatures (cold 4 degrees and warm 40 degrees C) in urinary bladder on rate of renal urine formation. The volume of fluid (isotonic saline) infused was 100 ml in all the cases. Presence of alkaline saline (pH-9 and pH-10) and warm saline in urinary bladder, decreased the rate of urine flow whereas acidic and cold saline increased the rate of urine flow significantly. (P < 0.05 and < 0.0001 respectively). The observed effects disappeared when 4% xylocaine in 100 ml saline was infused and evacuated prior to infusion of fluids of different pH and temperature. The observation suggests existence of chemosensitive and thermosensitive mechanisms in the vesical mucosa regulating urine formation by the kidney.
Subject(s)
Animals , Dogs , Feedback , Female , Hydrogen-Ion Concentration , Male , Temperature , UrinationABSTRACT
The present study was undertaken to investigate the effect of the presence of solutions of different osmolality (hypertonic 3%, 4% and 5% hypotonic 0.1%, 0.2% and 0.3% in the urinary bladder on renal urine formation. The study was conducted on 36 dogs of either sex. 100 ml normal saline was used for distending the bladder. Various hypertonic and hypotonic solutions were infused inside the bladder and rate of urine formation was recorded. Presence of hypertonic fluid increased the rate of urine formation and hypotonic fluid decreased it.